ARF1 antibody - ADP-ribosylation factor 1 AS08 325 from Agrisera

Product Specs
ItemARF1 antibody - ADP-ribosylation factor 1
CompanyAgrisera
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAS08 325
ApplicationsImmunofluorescence, Immunogold, Western blot
ClonalityPolyclonal
ImmunogenRecombinant GST fusion of full length of Arabidopsis thaliana ARF1 (P36397, AT2G47170)
HostRabbit
Quantity50 µl
FormatLyophilized
TargetARF1
Research AreaCell Biology
NCBI Gene AliasesT8I13.1, ADP-RIBOSYLATION FACTOR, BEX1, bfa-visualized exocytic trafficking defective 1
ReactivityAr, Chlamydomonas, Pl, Potato, Rice, Tobacco
PuritySerum
StorageStore lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
Molecular Weight21 kDa (Arabidopsis thaliana)

Specifications/Features

1:1000 with standard ECL (WB), 1: 1000 (IF). 1: 100 (IG)

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Agrisera

Box 57

Vännäs

Sweden

Phone: +46 (0) 935 33033

Email: info@agrisera.com
orders@agrisera.com

Company Profile

Website: http://www.agrisera.com

(1) Uptake of oomycete RXLR effectors into host cells by clathrin-mediated endocytosisThe Plant CellJune 26, 2023Haixia Wang, Shumei Wang, Wei Wang, Lin Xu, Lydia R J Welsh, Marek Gierlinski, Stephen C Whisson, Piers A Hemsley, Petra C Boevink, Paul R J Birch(5f8-100, Chromotek) raised in rat at 1:2,000 dilution; and anti-Toc75 (AS08 351, Agrisera), anti-Arf1 (AS08 325, Agrisera), anti-calnexin (AS12 2365, Agrisera), and anti-UGPase (AS05086, Agrisera) raised in rabbit

(2) Phosphate transporter PHT1;1 is a key determinant of phosphorus acquisition in Arabidopsis natural accessionsPlant PhysiologyAugust 29, 2022Pei-Shan Chien, Ya-Ting Chao, Chia-Hui Chou, Yu-Ying Hsu, Su-Fen Chiang, Chih-Wei Tung, Tzyy-Jen Chiou2011). ADP-ribosylation factor 1 (Arf-1), a Golgi marker, recognized by anti-Arf-1 antibody (Agrisera, AS08 325), was used as a loading control. The chemiluminescence signal was acquired by an UVP BioSpectrum 815

(3) Small paraquat resistance proteins modulate paraquat and ABA responses and confer drought tolerance to overexpressing Arabidopsis plantsPlant, Cell & EnvironmentJuly 1, 2022Dóra Faragó, Laura Zsigmond, Dániel Benyó, Rubén Alcazar, Gábor Rigó, Ferhan Ayaydin, Sahilu Ahmad Rabilu, Éva Hunyadi‐Gulyás, László Szabadosto detect the GFP‐ or HA‐tagged SPQ proteins, respectively. ARF1 was detected with antibody Agrisera AS08325 and Agrisera AS10710 was used to detect histone H3. After washing with 1×TBST three times for 10 min,

(4) Systematic analysis of specific and nonspecific auxin effects on endocytosis and traffickingPlant PhysiologyJune 11, 2021Madhumitha Narasimhan et al.following primary and secondary antibodies were used: rabbit anti-ARF1 1:500 (Agrisera, Vännäs, Sweden, AS08325), goat anti-PIN1 1:600 (SantaCruz Technologies, San Diego, CA, sc-27163), mouse anti-GFP 1:500 (Sigma,

(5) SYNERGISTIC ON AUXIN AND CYTOKININ 1 positively regulates growth and attenuates soil pathogen resistanceNature CommunicationsDecember 1, 2020Andrej Hurný et al.(Agrisera AS09481), rabbit anti-SEC21 (1:800) (Agrisera AS08327), rabbit anti-ARF1 (1:600) (Agrisera AS08325), rabbit anti-SYP61 (1:200)89, rabbit anti-ECH (1:600) (kindly provided by R.P. Bhalerao, Umea Plant

(6) Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin TransportersThe Plant CellMay 1, 2020Xixi Zhang et al.1:1,000 (v/v), rabbit anti-PIN2 (Abas et al., 2006) diluted 1:1,000 (v/v), rabbit anti-ARF1 (cat. no. AS08 325; Agrisera; Pimpl et al., 2000) diluted 1:1,000 (v/v), and rabbit anti-gCOP (cat. no. AS08 327; Agrisera;

(7) Homologous Proteins of the Manganese Transporter PAM71 Are Localized in the Golgi Apparatus and Endoplasmic ReticulumPlants (Basel, Switzerland)February 13, 2020Hoecker N, Honke A, Frey K, Leister D, Schneider A.A6455, 1:2000 dilution), SMT1 (Agrisera AS07 266, Vannas, Sweden; 1:500 dilution) and ARF1 (Agrisera AS08 325, 1:1000 dilution) in combination with anti-rabbit IgG horseradish peroxidase (HRP) (Sigma Aldrich, St.

(8) Cilia-based peptidergic signalingPLOS BiologyDecember 6, 2019Raj Luxmi, Dhivya Kumar, Richard E. Mains, Stephen M. King, Betty A. Eipperusing Gene Tools software from Syngene (Frederick, MD). Rabbit Arf1 antibody was from Agrisera (cat# AS08 325; Vannas, Sweden). CrPHM and CrPAL enzyme activities in the soluble fractions (0.25 μg protein/ sample)

(9) Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport ProteinsFrontiers in Plant ScienceMay 15, 2019Liuqing Kuang, Shangwu Chen, Yan Guo, Huiqin Ma73.5–80 kDa; Agrisera, AS09 614, 1:2000), ARF1 (ADP-ribosylation factor 1; Golgi marker, 21 kDa; Agrisera AS08 325, 1:1000), AOX1/2 (plant alternative oxidase 1 and 2; mitochondrial marker, 36–40 kDa; Agrisera AS04 054

(10) A single class of ARF GTPase activated by several pathway-specific ARF-GEFs regulates essential membrane traffic in ArabidopsisPLOS GeneticsNovember 15, 2018Manoj K. Singh et al.

(11) Multifaceted plant responses to circumvent Phe hyperaccumulation by downregulation of flux through the shikimate pathway and by vacuolar Phe sequestrationThe Plant journal : for cell and molecular biologyDecember 1, 2017Lynch JH, Orlova I, Zhao C, Guo L, Jaini R, Maeda H, Akhtar T, Cruz-Lebron J, Rhodes D, Morgan J, et al.

(12) A genome-scale analysis of mRNAs targeting to plant mitochondria: upstream AUGs in 5' untranslated regions reduce mitochondrial associationThe Plant journal : for cell and molecular biologyDecember 1, 2017Vincent T, Vingadassalon A, Ubrig E, Azeredo K, Srour O, Cognat V, Graindorge S, Salinas T, Maréchal-Drouard L, Duchêne AM.

(13) Enrichment of Golgi Membranes from Triticum aestivum (Wheat) SeedlingsIsolation of Plant Organelles and StructuresJanuary 1, 2017Wei Zeng, Berit Ebert, Harriet T. Parsons, Carsten Rautengarten, Antony Bacic, Joshua L. Heazlewoodcontinuous sucrose gradient. Organelle marker antibodies are: Arf1 (ADP-ribosylation factor 1, Agrisera AB, AS08 325); CRT1 (calreticulin, Abcam, ab2907); PsbA (D1 protein of PSII, Agrisera AB, AS01 016); VDAC1 (voltagedependent

(14) Biogenesis of phased siRNAs on membrane-bound polysomes in ArabidopsiseLifeDecember 12, 2016Shengben Li et al.RRID:AB_10013427; anti-HSC70: Enzo Life Sciences Cat# ADI-SPA-818-F RRID:AB_11180122; anti-ARF1: AgriSera Cat# AS08 325 RRID:AB_1271007; anti-SYP22: a gift from Dr. Natasha Raikhel; PEPC: AgriSera Cat# AS09 458 RRID:AB_2063166).

(15) Phosphatidylserine Synthase Controls Cell Elongation Especially in the Uppermost Internode in Rice by Regulation of ExocytosisPLOS ONEApril 7, 2016Jin Ma et al.

(16) The Qb-SNARE Memb11 interacts specifically with Arf1 in the Golgi apparatus of Arabidopsis thalianaJournal of experimental botanyNovember 1, 2015Claireline Marais, Valérie Wattelet-Boyer, Guillaume Bouyssou, Agnès Hocquellet, Jean-William Dupuy, Brigitte Batailler, Lysiane Brocard, Yohann Boutté, Lilly Maneta-Peyret, Patrick Moreau

(17) UDP-D-galactose synthesis by UDP-glucose 4-epimerase 4 is required for organization of the trans-Golgi network/early endosome in Arabidopsis thaliana root epidermal cellsJournal of plant researchSeptember 1, 2015Sheliang Wang, Toshiaki Ito, Masataka Uehara, Satoshi Naito, Junpei Takano

(18) High lipid order of Arabidopsis cell‐plate membranes mediated by sterol and DYNAMIN‐RELATED PROTEIN1A functionThe Plant JournalDecember 1, 2014Márcia Frescatada‐Rosa, Thomas Stanislas, Steven K. Backues, Ilka Reichardt, Shuzhen Men, Yohann Boutté, Gerd Jürgens, Thomas Moritz, Sebastian Y. Bednarek, Markus Grebe

(19) The Contractile Vacuole as a Key Regulator of Cellular Water Flow in Chlamydomonas reinhardtiiEukaryotic CellNovember 1, 2014Karin Komsic-Buchmann, Luisa Wöstehoff, Burkhard Becker

(20) Arabidopsis WAT1 is a vacuolar auxin transport facilitator required for auxin homoeostasisNature CommunicationsDecember 1, 2013Philippe Ranocha et al.

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Anti-ARF1 : ADP-ribosylation factor 1

ARF1 antibody - ADP-ribosylation factor 1 from Agrisera

Description

Specifications/Features

Agrisera

Citations (20)

Reviews

ARF1 antibody - ADP-ribosylation factor 1 from Agrisera

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